Variant Calling: The Ultimate Tutorial

From raw sequencing data to variant calling and annotation

This step-by-step tutorial will walk you through variant calling and variant annotation. You'll learn how to fetch whole-genome sequencing data, perform quality control and read mapping, and call small variants (i.e. SNPs and indels), before finally visualizing and annotating the obtained variants.

We’ll provide everything you need to follow along, including detailed instructions, scripts, estimated run times, and a guide to interpreting the results. All that's required is some very basic knowledge of Linux/Unix-like operating system commands and a regular laptop. Let's get started!