ATAC-Seq: The Ultimate Tutorial

From raw data to differential chromatin accessibility analysis

This end-to-end tutorial will guide you through every step of ATAC-seq data analysis. We’ll show you how to set up your computing environment, fetch the raw sequencing data, perform read mapping, peak calling, and differential accessibility analysis, and finally visualize and interpret the results.

All you need is a reasonably modern desktop/laptop computer with a Linux/Unix-like operating system and some basic knowledge of bash and R scripting. Let's get started!