RNA-Seq: The Ultimate Tutorial

From raw sequencing data to differential gene expression analysis

This tutorial will show you how to perform a differential gene expression analysis using RNA sequencing (RNA-Seq) data. Our goal is to make RNA-Seq accessible for everyone by using plain language and providing every tool and script you need along the way. We’ll also point you to more extensive reading, so you can dig deeper into the RNA-Seq methodology.

To follow along, all you need is a modern desktop or laptop computer with a Linux/Unix-like operating system and some basic understanding of shell and R scripting. Let’s get started!